Title Modular teaching and learning offers for contemporary and needed further education
Project Number D/04/B/F/PP-146 157
Product Preparation of nanostrctured thin-gilm interfaces applicable in biosebsors
Title Preparation of nanostrctured thin-gilm interfaces applicable in biosebsors
Product Type modules
We have investigate the effect of gold nanostructured surface electrode on the interaction between dsDNA and its redox marker complex Co[(phen)3]3+, by using differential pulse voltammetry, cyclic voltammetry with the K3[Fe(CN)6] indicator, guanine moiety electrochemical oxidation and impedance measurements have been performed too.
Target group univerisity, research center
Microroughness and morphplogy of Au thin film surfaces indicate that the finest nanocrystaline surafces were obtained by both simple sputtering (sample Au 1) and ion etching 10 min/150 °C after sputtering (sample Au 2). The highest roughness and surfase grain structure of Au thin film sputtered in bias mode (sample Au 4) were caused particularly by atomic-scale heating of growing film as an effect of energetic ion bombardment.
Initial etching at higher temperature did not modify density of active centre, significantly. At longer time of etching (20 min.) microroughness of surface grows considering anisotropy of ion etching, on the other side the number of active centres decreases. Dissociation rate constant indicate any differences in the DNA structural arrangement and marker binding ability at the Au sensors. The stable marker binding is of great interest for the detection schemes using the electrode transfer. The difference between the CV profiles for these four Au electrode could be caused by various methods of preparation the electrodes.
Results demonstrated that gold nanostructured thin-film give the better possibility for the detection of the presence and state of the DNA in comparison with screen-printed carbon electrodes.
Area of application